PCR and LacOperon

| March 27, 2015

Paper, Order, or Assignment Requirements

 

This is a lab report that will be combining two labs I will attach both of the labs protocols and the outline of the the lab report the highlighted sections require the results and I will complete those.

SECTION B: PCR Amplification of Alu Sequences

 

Questions:

Q1) What was the annealing temperature that was programmed into our thermocycler? Why was this temperature chosen?

 

Q2) Calculate the melting temperature of each of the following primers;

 

Forward Primer         5’-GGA TCT CAG GGT GGG TGG CAA TGC T-3’

Reverse Primer        5’-GAA AGG CAA GCT ACC AGA AGC CCC AA-3’

 

Tm = [2°C x (A+T)] + [4°C x (G+C)]

 

Show your calculations.

 

ACTIVITY B2:PCR Movie or online animations

 

  1. Watch the PCR movie at http://youtu.be/iQsu3Kz9NYo
  2. After watching the movie, answer the following three questions.

 

Questions:

 

Q1.  After which PCR cycle do you obtain a target sequence of the appropriate sizes

 

Q2.  Starting with 1 DNA template molecule, how many copies of the target DNA do you obtain after 25 cycles?  And 35 cycles?

 

Q3.  Starting with 1 DNA template molecule, how many variable-length DNA fragments do you obtain after 25 cycles?  And 35 cycles?

 

 

WEEK 2:

 

 

SECTION C: Electrophoresis of Amplified DNA

 

Comprehension Questions:

Q1. What is a polymorphism?

 

 

Q2. How is Taq DNA polymerase different from DNA polymerase found in E. coli?

 

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Category: Biology

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