Coyne College Seed Germination Lab Report Would you expect endangered species to be more frequently generalists or specialists? Explain your answer.How doe

Coyne College Seed Germination Lab Report Would you expect endangered species to be more frequently generalists or specialists? Explain your answer.How does temperature affect water availability in an ecosystem?Choose a species and describe some adaptations that species developed that allow them to survive in their native habitat Name
Prof.
Date
Pre-Lab Questions
1. Would you expect endangered species to be more frequently generalists
or specialists? Explain your answer.
2. How does temperature affect water availability in an ecosystem?
3. Choose a species and describe some adaptations that species developed
that allow them to survive in their native habitat
©eScience Labs, 2016
Experiment 1: Effects of pH on Radish Seed Germination
Natural soil pH depends on the parent rock material from which it was formed
and processes, such as climate. Soil pH is a measure of the acidity or alkalinity
of the soil. Acidic soils are considered to have a pH of 5.0 or lower, whereas 10.0
or above is considered a strong basic or alkaline soil. The pH of soil affects the
solubility of nutrients in soil water. It therefore also affects the amount of nutrients
available for plant uptake. Different nutrients are available under differing pH
conditions.
In this experiment, we will look at the effect of pH on the germination and growth
rate of radish seeds to determine the range of pH tolerance for the seed. Acidic
or basic water will be used to stimulate acidity or alkalinity in soil.
Materials
2 mL 4.5% Acetic Acid (Vinegar),
C2H4O2
Permanent Marker
(3) 5 cm Petri Dishes
3 pH Test Strips
Radish Seed Packet
Ruler
2 mL 15% Saturated Sodium
Bicarbonate
(Baking Soda) Solution, NaHCO3
*Paper Towel Sheets (cut to fit
into the petri dish)
*Scissors
*Sunny Location
*Water
*You Must Provide
Procedure
1. Use the permanent marker to label the top of each of the three petri
dishes as Acetic Acid, Sodium Bicarbonate, or Water.
2. Carefully cut three small circles from the paper towel sheets. The circles
should comfortably fit within the bottom of the petri dish.
3. Place the circles in the dishes and wet them with approximately 2 mL of
the respective solution (acetic acid, sodium bicarbonate, or water).
©eScience Labs, 2016
4. Gently press the reaction pad of three pH test strips onto the wet paper
towels. Record your data in Table 1.
5. Arrange 10 radish seeds on each paper towel in each petri dish. Make
sure the seeds have space and are not touching. Then, place the top of
the petri dish on the bottom.
6. Create a hypothesis regarding which environment will provide the
heartiest radish seed growth. Be sure to indicate why you believe this will
be true using scientific reasoning. Record your hypothesis in the post-lab
questions.
7. Place the petri dishes in a sunny or well-lit, warm place. Be sure to keep
the paper towels moist for the length of the experiment with the
appropriate solution if any of the towels dry out.
8. Observe the seeds daily for seven days, and record the number of seeds
that germinate in Table 1. Note when the seeds crack and roots or shoots
emerge. On the seventh day, record the lengths of radish seed sprouts
(mm or cm).
Table 1: pH and Radish Seed Germination
Stage/Day Observations
Acetic Acid
Sodium Bicarbonate
Water
Initial pH
1
2
3
4
5
6
7
©eScience Labs, 2016
Post-Lab Questions
1. Record your hypothesis from Step 6 here:
2. Construct a line graph based on the data from Table 1 in the space below.
Place the day on the x-axis, and the number of seeds germinated on the
y-axis. Be sure to include a title, label the x and y axes, and provide a
legend describing which line corresponds to each plate (e.g., blue = acetic
acid, green = sodium bicarbonate, etc.).
3. Was there any noticeable effect on the germination rate of the radish
seeds as a result of the pH? Compare and contrast the growth rate for the
control with the alkaline and acidic solutions.
4. According to your results would you say that the radish has a broad pH
tolerance? Why or why not? Use your data to support your answer.
5. Knowing that acid rain has a pH of 2-3 would you conclude that crop
species with a narrow soil pH range are in trouble? Is acid rain a problem
for plant species and crops?
6. Research and briefly describe a real world example about how acid rain
affect plants. Be sure to demonstrate how pH contributes to the outcome,
and proposed solutions (if any). Descriptions should be approximately 2 3 paragraphs. Include at least three citations (use APA formatting).
©eScience Labs, 2016

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